Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.
Identifieur interne : 001210 ( Main/Exploration ); précédent : 001209; suivant : 001211Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.
Auteurs : Sébastien Balme [Suisse] ; Fazil O. Gülaçar [Suisse]Source :
- Food chemistry [ 0308-8146 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Phytosterols.
- chemistry : Citrus sinensis.
- methods : Gas Chromatography-Mass Spectrometry, Solid Phase Microextraction.
- Beverages, Molecular Structure.
Abstract
The potential of solid-phase microextraction on polyacrylate coated fibre, with sequential or simultaneous trimethylsilyl derivatisation followed by gas chromatographic-mass spectrometric analysis, was evaluated for a rapid determination of the distribution of the phytosterols in aqueous food matrixes. Influences of different parameters (bis(trimethylsilyl)trifluoro-acetamide and sterol exposure time, sterol concentration and experimental protocol) on the recovery of sterols were investigated to determine optimum conditions which were tested for sterol extraction and analysis from orange juice. Best selectivity, sterol recovery and derivatisation yields were obtained by extraction and simultaneous derivatisation through immersion of the SPME-PA fibre in the orange juice (10min, 65°C) after headspace absorption of BSTFA (30min, 65°C) on the fibre. Nevertheless the method developed cannot be used for quantitative analysis. But the possibility to effect rapid screen of phytosterol containing in complex media have been shown.
DOI: 10.1016/j.foodchem.2011.10.097
PubMed: 26434339
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000612
- to stream PubMed, to step Curation: 000612
- to stream PubMed, to step Checkpoint: 000612
- to stream Ncbi, to step Merge: 001D33
- to stream Ncbi, to step Curation: 001D33
- to stream Ncbi, to step Checkpoint: 001D33
- to stream Main, to step Merge: 001222
- to stream Main, to step Curation: 001210
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.</title><author><name sortKey="Balme, Sebastien" sort="Balme, Sebastien" uniqKey="Balme S" first="Sébastien" last="Balme">Sébastien Balme</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4, Switzerland. Electronic address: sebastien.balme@univ-montp2.fr.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Gulacar, Fazil O" sort="Gulacar, Fazil O" uniqKey="Gulacar F" first="Fazil O" last="Gülaçar">Fazil O. Gülaçar</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4, Switzerland. Electronic address: fazil.gulacar@chiphy.unige.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:26434339</idno><idno type="pmid">26434339</idno><idno type="doi">10.1016/j.foodchem.2011.10.097</idno><idno type="wicri:Area/PubMed/Corpus">000612</idno><idno type="wicri:Area/PubMed/Curation">000612</idno><idno type="wicri:Area/PubMed/Checkpoint">000612</idno><idno type="wicri:Area/Ncbi/Merge">001D33</idno><idno type="wicri:Area/Ncbi/Curation">001D33</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D33</idno><idno type="wicri:doubleKey">0308-8146:2012:Balme S:rapid:screening:of</idno><idno type="wicri:Area/Main/Merge">001222</idno><idno type="wicri:Area/Main/Curation">001210</idno><idno type="wicri:Area/Main/Exploration">001210</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.</title><author><name sortKey="Balme, Sebastien" sort="Balme, Sebastien" uniqKey="Balme S" first="Sébastien" last="Balme">Sébastien Balme</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4, Switzerland. Electronic address: sebastien.balme@univ-montp2.fr.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Gulacar, Fazil O" sort="Gulacar, Fazil O" uniqKey="Gulacar F" first="Fazil O" last="Gülaçar">Fazil O. Gülaçar</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4, Switzerland. Electronic address: fazil.gulacar@chiphy.unige.ch.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Mass Spectrometry, University of Geneva, Sciences I, Boulevard d'Yvoy 16, CH-1211 Geneva 4</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author></analytic><series><title level="j">Food chemistry</title><idno type="ISSN">0308-8146</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Beverages</term><term>Citrus sinensis (chemistry)</term><term>Gas Chromatography-Mass Spectrometry (methods)</term><term>Molecular Structure</term><term>Phytosterols (chemistry)</term><term>Solid Phase Microextraction (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Phytosterols</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gas Chromatography-Mass Spectrometry</term><term>Solid Phase Microextraction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Beverages</term><term>Molecular Structure</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The potential of solid-phase microextraction on polyacrylate coated fibre, with sequential or simultaneous trimethylsilyl derivatisation followed by gas chromatographic-mass spectrometric analysis, was evaluated for a rapid determination of the distribution of the phytosterols in aqueous food matrixes. Influences of different parameters (bis(trimethylsilyl)trifluoro-acetamide and sterol exposure time, sterol concentration and experimental protocol) on the recovery of sterols were investigated to determine optimum conditions which were tested for sterol extraction and analysis from orange juice. Best selectivity, sterol recovery and derivatisation yields were obtained by extraction and simultaneous derivatisation through immersion of the SPME-PA fibre in the orange juice (10min, 65°C) after headspace absorption of BSTFA (30min, 65°C) on the fibre. Nevertheless the method developed cannot be used for quantitative analysis. But the possibility to effect rapid screen of phytosterol containing in complex media have been shown.</div></front></TEI><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Genève</li></region><settlement><li>Genève</li></settlement><orgName><li>Université de Genève</li></orgName></list><tree><country name="Suisse"><region name="Canton de Genève"><name sortKey="Balme, Sebastien" sort="Balme, Sebastien" uniqKey="Balme S" first="Sébastien" last="Balme">Sébastien Balme</name></region><name sortKey="Gulacar, Fazil O" sort="Gulacar, Fazil O" uniqKey="Gulacar F" first="Fazil O" last="Gülaçar">Fazil O. Gülaçar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001210 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001210 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26434339
|texte= Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26434339" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |